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Image Search Results
Journal: Frontiers in Oncology
Article Title: Targeting BET Proteins With a PROTAC Molecule Elicits Potent Anticancer Activity in HCC Cells
doi: 10.3389/fonc.2019.01471
Figure Lengend Snippet: BETd-260 induces BET degradation, triggers apoptosis, and inhibits proliferation in HCC xenograft tissue in mice. BALB/c mice bearing HepG2 and BEL-7402 xenograft tumors were treated by a single intravenous dose of 5 mg/kg BETd-260 (BETd) for 24 h or vehicle (Veh). (A) Two to three mice were sacrificed and tumor tissue was harvested. The expression of BRD2, BRD3, and BRD4, Mcl-1, Bad, activated caspase-3, cleaved PARP, and Ki 67 was examined by immunohistochemistry staining. Representative photographs were presented. (B) The percentages of HCC tumor cells positively stained with BRD2, BRD3, and BRD4, Mcl-1, Bad, cleaved caspase-3 (c-Casp3), cleaved PARP (c-PARP), and Ki 67 were quantified under microscopy, and plotted. Data are representative of three independent experiments. ** p < 0.01.
Article Snippet: The following antibodies were used for IHC: BRD2 (IHC-00612), BRD4 (HC-00396),
Techniques: Expressing, Immunohistochemistry, Staining, Microscopy